Astelia Au

5 Questions 5 Answers 0 Followers

Questions related from Astelia Au

Why my target protein shows two elution peaks in the chromatogram during Ni-NTA?

I obtained two peaks during the affinity chrokatography which are next to each other. After I run SDS-PAGE, both peaks are my target protein. I am curious about the reason behind that, can anyone...

29 August 2019 3,592 3 View

Is there any possibilities that the salt crystal traps IZIT dye?

Is there any possibilities that the salt crystal traps IZIT dye? Besides, is there any possibilities that the protein crystal does not traps the IZIT dye? Thank you.

11 April 2019 7,956 1 View

Why thrombin doesn't show in SDS-PAGE after thrombin cleavage?

I have incubated my protein with thrombin, and it has successfully cleaved the tag from my protein. I was thinking that the thrombin should have show in the SDS-PAGE as well, but I didn't see any...

01 March 2019 10,075 10 View

Will the band of plasmid different from the expected base pair?

My plasmid size is ~6000bp, when I extracted the plasmid and run it in 1% agarose, it shows one band above 1kb, one band in between 1kb and 7000bp, and one band in between 5200bp - 4000bp. There...

01 November 2018 7,116 5 View

Hanging long strand in my predicted protein modeled resulted from unaligned sequence, what should I do?

From my alignment results, there is a consecutive sequence (around 32 amino acids) which is present in my target but not in my template. After I modeled my protein with MODELLER, that sequence...

29 January 2018 7,051 5 View