The ladder is transferred but not the high molecular weight proteins. What's wrong?

Hello. I need to detect a 100 KDa protein. I loaded 40 ug of samples in gel polyacrylamide 8%., SDS page in the mini gel-Biorad system, wet transfer (+20% methanol), 300 mA 1h. Nitrocellulose...

29 September 2020 1,124 8 View