Asrar Alam

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Questions related from Asrar Alam

How to lyse HEK293F cells to extract cytosolic recombinant proteins?

Hello, I am trying to purify His-tagged recombinant proteins expressed in cytosol from HEK293F cells from 200 ml culture. What could be the best method to lyse these cells to purify the proteins...

08 November 2024 6,502 7 View

Are there alternative ways to grow HEK293F cells if you do not have CO2 incubator equipped with shaker?

HEK293F cells grow in suspension culture and need constant shaking for growth that requires a CO2 incubator equipped with shaker. Has anyone tried alternative ways to culture these cells when...

09 January 2022 6,544 4 View