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I'm having trouble with my Western blot results, and I'm hoping to get some advice. I used a 4% stacking gel and an 8% resolving gel to detect GAPDH (36 kDa). The SDS-PAGE was run at 120V for 2...
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I’m currently running a Western blot to detect pSTAT3 (86 kDa) using a 10% resolving gel and a 4% stacking gel. Here's a summary of my protocol: Cell Lysis: I lyse 2 million cells using 45 µL...
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