Western Blot Bands Are Smudged, bent and Unclear - Potential Causes?

I'm having trouble with my Western blot results, and I'm hoping to get some advice. I used a 4% stacking gel and an 8% resolving gel to detect GAPDH (36 kDa). The SDS-PAGE was run at 120V for 2...

16 August 2024 9,945 3 View