Asmat Ullah

4 Questions 6 Answers 0 Followers

Questions related from Asmat Ullah

Is Anti-FLAG® M2 Magnetic Beads work for purification of 3x FLAG tagged protein ?

Can anyone share their experience of working with Anti-FLAG® M2 Magnetic Beads used for purification of 3x FLAG tagged protein.

26 May 2024 7,384 1 View

Does anyone know the cause of plant protein gets brown and precipitates quickly how to get rid of those colors and precipitation problem?

We have expressed our protein in chloroplast when we add extraction buffer it get precipitate soon after centrifugation as this is real quick even we don't have a time to prepare sample for...

11 January 2024 9,699 10 View

Polyethyleneimine is used for DNA precipitation after cell lysis during protein purification. Is there any alternative to precipitate nucleic acid ?

After lysis of bacterial cell whenever I went for centrifugation every time my supernatant(have protein of interest) got mixed with pellet even centrifugation at very high (g) and low temperature...

15 November 2021 5,016 3 View

Do you know how to prevent the precipitation of protein after concentrating using Amicon ?

Hi everyone, I have purified my bacterial protein weigh 22kda after purification I start concentrating it but after concentrating a few ml my protein start precipitating aven I have kept my ph 1...

04 August 2021 1,026 3 View