Asmara Imtiaz

3 Questions 8 Answers 0 Followers

Questions related from Asmara Imtiaz

Do extra nucleotides in a tagged protein, just before Aug of the proteins, affect the structure of the proteins?

My protein is cloned in pEt 41a vector. I have shifted it from pEt 21a to pEt 41a by designing the primers with Kpn site. So that we may remove the tag later on using thrombin. But in this way...

24 April 2022 4,981 2 View

My protein is Cadmium binding protein. Can I use Cadmium column instead of Nickel column for purification of protein via IMAC method?

I want to do structural analysis of the protein after purification.

20 February 2019 3,875 6 View

Metallothionein Protein purification

My Protein is Cadmium binding and around 25 KDa molecular weight. PI is 4. 76. Can anybody recommend me which column and chromatography should I use for protein purification. Its soluble protein....

01 January 1970 6,057 5 View