Asmaa Elrakaybi

3 Questions 3 Answers 0 Followers

Questions related from Asmaa Elrakaybi

Why do the concentrations of some samples with ODs less than the lowest standard still could be interpreted by my software?

Hello, I am using four parameter logistic (4-PL) curve fit (using GraphPad Prism8) to interpret my samples concentration after each ELISA assay. However, some of my samples with ODs less than the...

12 December 2019 2,282 3 View

How should be the CV% calculated in ELISA?

I am currently carrying out my ELISA assays and I want to calculate the CV% for each of my samples. Should the CV% be calculated based on the replicate OD values or based on replicate...

10 October 2019 377 4 View

Do I have to dilute my samples during ELISAs?

Good evening, I have done several ELISA assays for my biomarkers, and I have observed that for some assays I get different concentrations from kits of different LOT numbers (but still from the...

02 February 2019 9,329 8 View