Hi everyone, does anyone have experience in doing yeast two hybird with an insert ORF of ~7Kb. Please let me know.

I have basically two questions: 1. Which Taq polymerase system is reliable to get 7kb fragment from cDNA. The ORF of the gene is about 6.9 kb and it would then be used for yeast two hybrid in...

19 July 2017 9,402 6 View