I get truncated bands in the overexpression gel of my zif268 fusion nuclease protein. Is it because of the flexible linker?

Hi. The fuson protein is zif268- linker(12 aa long consisting of G and S)- protein. when I overexpress it in BL21 or RIL, I get truncated bands. The protein should come at 80 kDa but i am getting...

15 June 2025 7,768 0 View