Ashutosh Kumar Rai

3 Questions 2 Answers 0 Followers

Questions related from Ashutosh Kumar Rai

Do I use antibiotic for comparing the growth of wild type and its one of the mutant in liquid medium?

I have constructed one mutant having kanamycin cassette. For the growth comparison between wild type and mutant of same species, is it essential to add kanamycin antibiotic in liquid medium? In...

11 November 2015 3,870 2 View

How to perform tetrathionate reductase assay?

First time i have to carried out in Azospirillum brasilense Sp7.

05 May 2015 8,580 0 View

Can anyone suggest a standard protocol for running TGGE of ssRNA, for melting profile of RNA?

I have synthesized ssRNA by incorporating T7 promoter in a strand of DNA. After pcr I purified it. Next in vitro-transcription of template was done at 37 deg for 3 hours. 20ul reaction template:...

10 October 2013 8,654 1 View