Ashok Kumar

3 Questions 2 Answers 0 Followers

Questions related from Ashok Kumar

What could be probable problem in induction of Protein expression in pET-28a-c(+) vector ?

I ligated ORF of size 1.2 Kb in pET-28a-c(+) vector and cloned in DH5 alpha strain of E.Coli. Restriction release of 1.2 Kb was observed. later i transformed it in Expression strain BL-21 and...

18 December 2017 2,725 2 View

Will Any one suggest me best insilico tool for protein interaction studies?

I am working on some protein and would like to know about probable candidate protein interacting with my query. sequence and protein structure is available with me. Please suggest me some good...

30 November 2016 1,243 2 View

Can anyone help me in troubleshooting of Total RNA Isolation Using Trizol method from wheat leaf (Grain filling stage). ?

I am using freshly puchased ambion trizol reagent. i tried using the protocol provided but not getting intact bands. i thought problem can be due to other reagent and changed chloroform and...

12 May 2016 1,947 7 View