Ashok K Gupta

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Questions related from Ashok K Gupta

PBMC isolation of Monocytes shows different small cells?

I am isolating and culturing the monocytes derived from patient blood samples. The following step followed- 1. Blood + PBS (1:1) mixed and topped up on the histopaque layer. Centrifuged at 400 g,...

13 July 2022 5,700 7 View

Does any one work on monocyte-derived hepatocyte-like cells (MH cells)?

I am culturing the MH cells from patient blood. Following the protocol (Benesic A et al 2012) but cells are not adhering (or loosely adhere) with culture plates. Cells are observed in discarded...

14 March 2022 6,940 0 View