Ashlee Sun

2 Questions 3 Answers 0 Followers

Questions related from Ashlee Sun

Can I transform pENTR plasmid in DH5a instead of TOP10 competent cells that come with the kit?

I cloned my insert into pENTR vector using TOP10 competent cells. Now I have my plasmid (pENTR+insert) and would like to amplify this plasmid. So I just want to do a regular heat shock...

04 January 2017 7,215 3 View

Why does BsmBI digestion cause smear?

I've used BsmBI from NEB to digest LentiGuide-puro vector (for CRISPR) multiple times: 1ul BsmBI + 5ul buffer 3.1 + 2~3ug vector + water = 50ul system, 55C. I found that when the incubation time...

10 June 2016 4,504 14 View