Ashlee Liao

3 Questions 1 Answers 0 Followers

Questions related from Ashlee Liao

How frequent to change neuron culture media?

I am looking into protocols for culturing primary rat hippocampal neurons (no feeder layers). The standard protocol from ThermoFisher advises to replace 50% of the media every 2-3 days. However,...

19 February 2021 8,276 5 View

How to calibrate a T/C CO2 sensor in an air-jacketed CO2 incubator without an independent CO2 sensor?

I have an air-jacketed CO2 incubator and I need to calibrate its thermal conductivity CO2 sensor. However, I do not have a CO2 sensor that is independent of the incubator to measure the CO2...

01 February 2020 8,881 1 View

What is a good procedure to identify if the CO2 incubator is the source of contamination in my cell cultures?

I have read some vague descriptions about how to screen the CO2 incubator as the source of contamination. I saw recommendations to leave a plate of cell culture media in the incubator and view it...

13 January 2020 8,760 2 View