Ashish Bihani

3 Questions 7 Answers 0 Followers

Questions related from Ashish Bihani

Nuclear Matrix: After DNAse digestion and high salt wash of isolated nuclei, the DNAse gets stuck in the nuclear scaffold. Why?

I am preparing Nuclear Matrix, the insoluble filamentous fraction of the nucleus. Here are the steps: Isolate nuclei Digest DNA with dnase and detergent Extract in 400mM and 2M NaCl...

05 May 2017 5,550 2 View

How do I capture co-ordination complex intermediate of a phosphate transfer?

In my hypothesis, I have several phosphates from different molecules forming co-ordinate bonds with Magnesium ion. To determine structure of the intermediate by crystallography, I need to freeze...

09 September 2014 433 2 View

How can we build a molecule from scratch?

I need to build a phosphate (in silico) on a polypeptide structure (15AA) in order to see the effect of phosphorylation on the polypeptide. Please suggest a software/tool that can Can build the...

08 August 2014 6,652 9 View