Arup Acharjee

3 Questions 17 Answers 0 Followers

Questions related from Arup Acharjee

During BrDu immunofluorescence detection why rat brain coronal cryosections fall off soon after the 1N/2N HCl denaturation step gelatin subbed sides?

Currently I am processing BrdU labelled rat brains for immunofluorescent detection. The cryosections are of 25 µm. My concern is that soon after the 2N HCl denaturation step the sections fall off...

06 February 2019 3,013 4 View

What could have gone wrong in the 2Dgel where the protein (soluble fraction) seem to have aggregated at low and high pH?

I am new to 2D gel electrophoresis. I tried to follow the basics but the results have come as an absolute shocker. As a newbie my troubleshooting skill is as limited as my hands on skill in the...

21 July 2018 5,272 4 View

I am getting a PCR amplification from a brain region, albeit a faint signal, while in RNA in situ I am not. Is this normal?

The signal varies with physiological state and RNA in situ signals are present when the transcription is quite high. The probes are all working and the protocol is fully standardized Is it so that...

22 October 2014 3,714 3 View