Arunkumar Venkatesan

4 Questions 13 Answers 0 Followers

Questions related from Arunkumar Venkatesan

Anyone have experience in performing native immunoblot for cell supernatant IP samples?

Can anyone suggest a protocol to verify the oligomerization of secreted protein using immunoprecipitation and native immunoblot.

10 October 2017 4,513 2 View

How would you distinguish and measure divalent cation binding to a protein?

I have been working on a protein which have multiple sites for binding magnesium ions. These sites are present at different domains of the same protein. How would I measure the binding affinity of...

02 February 2017 4,076 6 View

Any suggestions for non-separation of phosphorylated proteins in the SDS-PAGE gel?

I am performing an in vitro kinase assay with a kinase and its substrate proteins. When I setup the assay and ran using 8% SDS-PAGE gels, I could see the samples only getting stacked but not...

06 June 2016 9,290 13 View

Why is the wild type protein structure unstable when compared to its point mutants?

I am bit new to bioinformatics and I am working on protein-protein docking studies. I have modelled a protein structure and 9 of its point mutants using homology modelling. The template has a...

05 May 2015 9,410 7 View