Is there a way to determine the run distance between a nicked circular/relaxed circular and a supercoiled form of plasmid when run on a agarose gel?

On running a plasmid DNA on 1% Agarose gel, three bands were observed referring to the nicked circular, linear and supercoiled forms. The run difference between these form depend on the plasmid...

12 December 2015 3,932 5 View