Arun Tapal

2 Questions 9 Answers 0 Followers

Questions related from Arun Tapal

How do I prevent protein self aggregation during purification and purify seed globulin?

Hi Iam purifying seed storage protein (Globulin). Extraction buffer: Tris-Hcl pH 8, containing 0.5M NaCl, 10mM mercaptoethanol, 1mM PMSF, 1mM EDTA. Elution buffer for gel filtration: Tris-HCl pH8...

08 August 2015 2,609 5 View

Gel filtration profile of protein in presence and absence of low ammonium sulphate gives different profile. What could be the reason?

Protein eluted from phenyl sepharose column when re-eluted in gel filtration, show two peaks (120kDa and 100kDa). Same protein eluted directly in gel filtration elute as single sharp peak...

08 August 2015 6,271 6 View