Artemiy Volkov

2 Questions 1 Answers 0 Followers

Questions related from Artemiy Volkov

Is HEPES (and other) buffer slightly out of recommended pH range still functional?

Hello. HEPES-containing buffer is known to be efficient as buffering system within 6.8-8.2 pH range. Does anyone have experience using a buffer slightly outside this range (e.g. 6.5/8.5 or more...

11 December 2024 2,123 3 View

Can more than one TetraHis antibody bind the long His tag?

Hello. TetraHis antibodies recognise 4xHis-tag in the protein. What if I have a long His-tag (e.g. 10xHis-tag) and I perform ELISA or Western Blot based on native PAGE? Is there a chance that two...

16 April 2024 6,979 1 View