Arpita Vats

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Scale bar absent in phylogenetic tree in MEGA 7.0?

I constructed a phylogenetic tree with MEGA 7.0 by using bootstrap (1000 replicates). But the final tree obtained lacked a scale bar, though it is there in the normal tree. What could be the...

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How to remove 3' A overhang from Taq polymerase amplified PCR fragment?

I have amplified few fragments with Taq polymerase. Now can i remove 3' A overhangs by amplifying again using Phusion DNA polymerase (High fidelity with 3'-5' exonuclease activity) ?

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