Arnold Au

4 Questions 5 Answers 0 Followers

Questions related from Arnold Au

Removal of Laemmli buffer from SDS PAGE samples?

Hi, I would like to remove the various detergents in the SDS sample buffer (Laemmli buffer) I'm attempting to use a Slide-A-Lyzer cassette from Thermo but the bromophenol dye doesn't seem to be...

07 March 2025 7,286 2 View

Why is my actin insoluble after lyophilization?

Hi, I reconstituted some lyophilized actin powder from the manufacturer, made aliquots and freeze dried the aliquots for long term storage. However, reconstituting them in the same buffer and at...

11 September 2023 5,782 1 View

No signal detected for purified protein control in Western blot of immunoprecipitation?

Hi, I'm running an immunoprecipitation experiment, trying to pulldown actin from some RAW 264.7 cell homogenates. I ran a Western blot with a 1:5000 dilution of biotinylated anti-actin...

03 May 2022 1,353 0 View

Thick lanes with no bands in immunoprecipitation experiment SDS PAGE?

Hi, I'm having some trouble running a basic immunoprecipitation experiment. I'm trying to pull-down actin from RAW cells, but all I get is a smear throughout the entire lane with no visible or...

05 April 2022 2,221 9 View