LacZ/ccdB counter-selection - any thoughts on a 2 counter-selectors strategy?

updated (2014.07.24) I m constructing a DNA cassette (with genes sacB, SmR, and LacZ), which will replace the galK region of an E. coli strain lacking LacZ after recombination. 1) Anyone has a...

05 May 2014 7,067 2 View