Can anybody suggest a detailed protocol to C-terminally tag an essential gene in Plasmodium falciparum using CRISPR/Cas9?

I have been trying this for weeks but I do not observe transgenic parasites emerge after drug selection. My protocol in brief is as follows: (1) Designed gRNAs as 5'-G-N19-NGG-3' to be inserted...

05 May 2017 4,458 1 View