Arnab Bose

6 Questions 11 Answers 0 Followers

Questions related from Arnab Bose

ShRNA mediated knockdown showing effects in the protein level but not in the RNA level, what might be the reason?

Hello everyone, I have been trying to standardize the knockdown of my gene of interest with the shRNA bought from DHARMACON, which targets the coding sequences. Though I get reproducible results...

27 May 2015 9,780 6 View

G2-M blocking of mammalian cells?

I went through papers in which people had carried out thymidine block followed by nocodazole block for synchronizing cells in the G2-M phase. However, after the thymidine block for 24 hrs,a 3 hr...

02 July 2013 6,063 4 View

What is the approximate time for mammalian cells that have been synchronized for a particular phase to become asynchronous again?

Does it depend on the phase in which it has been blocked, i.e. G0/G1 will take one time while G2/M will have another? Does it depend on the cell's type (dividing cells) or the cells lines one is...

30 May 2013 8,349 3 View

Flag tagged clone not expressing.

I had cloned my gene of interest (GOI) into pCMV10-3X-Flag vector for expressing into mammalian cells. Even though the gene has been cloned and sequence confirmed, I am having a problem with...

07 May 2013 3,299 9 View

Finding new interacting partners for my protein of interest (POI).

I want to find new interacting partners for my protein of interest(POI), but I do not want to follow the more conventional and time taking methods like the yeast two hybrid system which also may...

19 January 2013 304 8 View

What causes a protein to get degraded or loose its activity if stored for a long period at 4 degrees?

I had purified a kinase of my interest to near homogeneity and had stored it at 4 degrees for approximately a month to carry out assays with it. However, I found the kinase activity to have been...

10 January 2013 5,343 10 View