Aritra Misra

4 Questions 6 Answers 0 Followers

Questions related from Aritra Misra

Fibronectin staining of deep tissue. Any advice?

I am performing fibronectin staining on Xenopus embryonic tissue. The tissue is fixed overnight in 4%PFA, permeabilized in 1xPBS with 0.3%TritonX, Blocked in 10%Blocking in 1XPBS with 0.3%TritonX,...

19 May 2021 3,435 1 View

What is the best tool to identify and provide a good pictorial representation of homologous DNA sequences between species?

I want to identify homologous DNA sequences between mouse chicken, xenopus and zebrafish for a human non coding genomic regions. Any suggestion of which tool to use. I would prefer the one that...

04 April 2018 2,635 3 View

Can we test the concept of "Specification" ?

Classical definition of SPECIFICATION says:"cell type is not yet determined and any bias the cell has toward a certain fate can be reversed or transformed to another fate"Do you agree to this...

20 December 2015 9,315 1 View

Any advice on why am I not able to detect input protein (47kDa) of a ChIP experiment in Western Blot?

I am doing ChIP of a transcription factor (47kDa) in differentiated hES cells. For the experiment I am using Millipore ChIP kit. We have earlier seen by western blot and immunostaining that upon...

24 November 2015 3,567 10 View