Arijit Ghosh

8 Questions 23 Answers 0 Followers

Questions related from Arijit Ghosh

Is there any tool or package to prepare input files for Circos from NGS data?

Any tool or package to prepare input files for Circos visualization of NGS data? I know of some, but not very user-friendly.

11 September 2018 716 2 View

Variant discovery for variation in quantitative traits between two lab selected populations (4 replicates) from pooled genome sequencing?

Is it possible to discover the variants responsible for variation in quantitative traits between two laboratory selected populations by pooled whole genome sequencing? If yes, how? Also, keep in...

04 September 2018 1,423 12 View

Is there any database to find a list of disease causing mutations of a specific protein?

I want to find out specific mutations which cause mutations in a specific protein, causing disease in humans. Is there any database for that?

24 May 2015 3,950 3 View

How can I measure intracellular Cadmium, Strontium and Arsenic concentration?

I want to measure the uptake of Cadmium, Strontium and Arsenic by cells. I want to do it in live cells mainly, so it will be better if someone can suggest any methods involving microscopy. Any...

10 December 2014 6,286 8 View

Can anyone help me with the differentiation of RAW 264.7 cells to osteoclasts? I'm using RANKL from R&D, carrier free. 50 ng/ml concentration?

I can not see any osteoclasts in my culture till now. I have done a TRAP assay twice, but there is no sign of any osteoclast after 7 days. The media was changed every three days. Please help me if...

27 November 2014 6,822 20 View

Which is the best protocol to use for isolating sperm from D.melanogaster?

I would like to get D.melanogaster sperm and image it for which I would like know how to segregate seminal vesicles from virgin Drosophila males.

27 June 2013 4,762 0 View

What are the working concentrations of Lat A and Lat B in Drosophila embryos for disrupting Actin completely?

Please provide reference.

19 June 2013 5,674 0 View

What can I use to permeabilize the vitelline membrane of a drosophila embryo, besides D-Limonene?

What can we use except D-limonene? It's basically an organic solvent, but the permeability is actually about the waxy layer (~5 nm) surrounding the vitelline membrane. Would acetone be right for this?

18 May 2013 500 12 View