Arif Istiaq

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Questions related from Arif Istiaq

Why do cell make sphere ? what's the mechanism of sphere formation for stem or differentiated cell ?

Sphere formation is known among normal or cancer stem cells. But whats the underlying mechanism of cells to come together and make sphere?

06 June 2019 9,484 4 View

What type of expression vector should be the best fit for an insert of ~3.1kb?

The desired gene to be expressed is aioBA of ~3.1kb . I have already tried pET100/D Topo vector. The insert was PCR amplified (forward primer with CACC overhang at 5’-end) with Pfu Polymerase (to...

06 June 2018 9,303 2 View