Arif Ashraf

3 Questions 10 Answers 0 Followers

Questions related from Arif Ashraf

Building reference genome for snpEff?

I’ve been having following issues while building my reference genome in snpEff. 1. It is suggested to change the configuration file. No matter, where I’m putting the information about my...

18 September 2019 9,467 1 View

What is the easier way to obtain Maize HapMap file?

I've been using Zea_mays.B73_RefGen_v4.dna.toplevel as reference genome for my analysis. At the same time, I need to use maize HapMap file? Does anyone used B74 RefGenv4 and HapMap3? I'm having...

04 September 2019 9,920 3 View

How to reduce the bursting of protoplast after transformation?

I'm isolating maize protoplast from etiolated leaf and transforming with the combination of plasmids to check the TF activation, for instance, construct with TF, construct with reporter. Most of...

04 June 2019 9,678 9 View