Aries Huang

3 Questions 5 Answers 0 Followers

Questions related from Aries Huang

Why RNA and protein concentrations difference big even they were from the same sample?

Hi all, Last week, I seeded cells from the same sample into 4 wells (fibroblasts; 300000 cells/well; 1ml/well). well-1 and well-2 are for qRT-PCR test; well-3 and well-4 are for Western blot...

10 June 2024 2,366 3 View

ki67 proliferation assay, starvation or not ?

Hi everyone, Recently I have been performing ki67 proliferation assay to determine the effects of TGFbeta1 on fibroblasts. My supervisor told me that I need to starve the fibroblasts for at least...

15 May 2024 2,361 2 View

Hi all, I used water as my negative control in my immunoblot experiment, but i can see a band in this lane, could any one tell me why?

Hi all, I used water as my negative control in my immunoblot experiment, but i can see a band in this lane, could any one tell me why.

07 May 2024 7,173 5 View