Ariel Dotts

2 Questions 1 Answers 0 Followers

Questions related from Ariel Dotts

Can I perform immunohistochemistry on tissue after it has already been flash frozen?

I did not plan on staining but now it is a necessity. So tissue was immediately frozen using liquid nitrogen and then placed in -80 C after removal. This is human tissue and I would like to stain...

09 October 2017 4,457 3 View

How do I determine which vector to use for site-directed mutagenesis?

I want to perform site directed mutagenesis to a sequence I have cloned into a pGL4.1 vector, but I'm not sure which vector to clone the mutants in once mutagenesis is validated. I want to clone...

28 September 2016 2,195 2 View