Arianna Ixcheel Celis

4 Questions 9 Answers 0 Followers

Questions related from Arianna Ixcheel Celis

What could be the reason for low recombinant protein yields from BL-21 E. coli cells?

I am having trouble obtaining a good amount of protein in order to carry out analysis on it. I have purified this protein before and obtained a good amount of it only growing 250mL of cells. Now...

04 April 2013 1,390 45 View

How can I make Tris-glycine gels for native gel electrophoresis analysis?

I am trying to perform a native gel electrophoresis assay on my protein. I made a 4-12% gradient gel just like I would make a denaturing gel, but omitted SDS. I ran the gel with a Tris-glycine...

04 April 2013 4,137 1 View

Can anyone provide advice on how to purify an S-tagged protein?

I am having trouble purifying my S-tagged protein. All protocols I have read call for batch mode, but say that column mode is also doable. I am using a column with S-protein Agarose from Novagen,...

01 January 2013 8,824 6 View

Does anyone have any suggestions on how to prevent proteolysis during purification of a His-tagged protein?

I am currently in the process of purifying a His-tagged protein. I use a Nickel column and then an ion exchange column to further get rid of other proteins. I have been pretty successful, but I am...

08 August 2012 9,005 25 View