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Questions related from Arianna Ixcheel Celis
I am having trouble obtaining a good amount of protein in order to carry out analysis on it. I have purified this protein before and obtained a good amount of it only growing 250mL of cells. Now...
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I am trying to perform a native gel electrophoresis assay on my protein. I made a 4-12% gradient gel just like I would make a denaturing gel, but omitted SDS. I ran the gel with a Tris-glycine...
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I am having trouble purifying my S-tagged protein. All protocols I have read call for batch mode, but say that column mode is also doable. I am using a column with S-protein Agarose from Novagen,...
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I am currently in the process of purifying a His-tagged protein. I use a Nickel column and then an ion exchange column to further get rid of other proteins. I have been pretty successful, but I am...
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