Arianna Ixcheel Celis

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Questions related from Arianna Ixcheel Celis

How do I make S.aureus small colony variant electrocompetent cells?

I am trying to perform complementation studies with Staph but I am having trouble making competent cells of my knock out strain. I think this might be due to the fact that this knock-out strain is...

23 November 2015 5,816 4 View

Can anyone provide advice on how to purify an S-tagged protein?

I am having trouble purifying my S-tagged protein. All protocols I have read call for batch mode, but say that column mode is also doable. I am using a column with S-protein Agarose from Novagen,...

02 January 2013 5,098 6 View

Does anyone have any suggestions on how to prevent proteolysis during purification of a His-tagged protein?

I am currently in the process of purifying a His-tagged protein. I use a Nickel column and then an ion exchange column to further get rid of other proteins. I have been pretty successful, but I am...

13 August 2012 4,042 24 View