When analyzing qPCR (SYBR green) results, how can I discriminate between absence of target gene or low presence when the Ct are high (33-36)?

I'm trying to detecte very small amounts of a mutant-type mtDNA using SYBR green qPCR. I can't really use a higher concentration of gDNA because my wild-type and housekeeping genes already have a...

15 October 2024 1,478 3 View