I am having trouble when purifying peptide on a semiprep column, the injections are not consistent. Any ideas?

I am purifying my peptides, easy linear 6 to 11 AA peptides, on a semi-prep column ( C18, 130A, 5um, 10 mmx250mm) but I am having trouble because my first injection is OK, but starting on the...

01 January 2016 965 3 View