Ari Simenauer

3 Questions 4 Answers 0 Followers

Questions related from Ari Simenauer

Stable knockdown with shRNA no difference in protein levels?

I've been trying to create a stable knockdown for months and months now with no success. I've used transduction and stable transfection with two different shRNA sequences targeting my gene of...

09 September 2018 8,979 3 View

Non-Reducing Conditions For Western Blot of Large Disulfide Linked Homo-dimer?

I am trying to perform a western blot to detect the S/T Kinase ATM in it's dimerized state. The 350 kDa homo-dimer is linked by 2 or more disulfide bridges, and I've detected the 175 kDa monomer...

02 February 2017 4,731 4 View

Protocol for treating cell culture with Doxorubicin Hydrochloride?

I am aiming to look at the phosphorylations state of the S/T kinase ATM under various conditions and as such need an activator as a control. Doxorubicin Hydrochloride seems to be the preferred non...

01 January 1970 7,150 2 View