Ari Gao

2 Questions 1 Answers 0 Followers

Questions related from Ari Gao

Why do dividing RPTECs show two different cluster morphologies?

We are currently culturing immortalized renal proximal tubules (RPTEC/TERT1) at passage 1. The cells are adherent, and so grow in clusters, but strangely, we observe two different types of...

25 June 2014 5,147 2 View

Does leaving (dilute) trypsin in with the cells during passage slow their growth?

I am growing hCMEC/d3 cells in Lonza EGM media with Bulletkit supplements. The passage protocol I use calls for just directly adding the resuspended cells, trypsin (.25% in EDTA) and all, to the...

04 March 2014 6,790 5 View