Arghya Sett

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Questions related from Arghya Sett

What could be the reason of very low cluster passing filter for my Aptamer pool Sequencing in MiSeq?

I have very low cluster passing filter for my SELEX pool Sequencing in Miseq using Nextera Miseq reagent kit V2 (300 cycles) and XT index kit set B. I performed all QC of pools after Adaptor and...

16 October 2024 4,526 0 View

How to remove background binding in Aptamer based ELISA?

I am using Thermo maxisorp 96 well immuno plates (https://www.thermofisher.com/order/catalog/product/437111) to attach whole virus particle. and then after incubation with FAM labelled aptamers, I...

04 October 2023 1,440 0 View

Can anyone help with the prediction of 3D structures of ss DNA /RNA aptamers?

What are the modelling tools/packages/softwares to predict ab-initio folding of single stranded oligonucleotide specially DNA Aptamers or RNA aptamers?

02 July 2014 605 2 View