Hi everyone. Does high concentrations of Tris-HCL buffer ( 2-3 mM Tris in 25 μl real time PCR reaction ) interfere with PCR reaction?

I have dissolved my primers in TE buffer 10X, which in the final PCR reaction will have an EDTA concentration of 0.02 mM and a Tris-HCL concentration of 2-3 mM. Will this interfere with the tag...

15 February 2022 6,668 3 View