Archita Ghoshal

4 Questions 12 Answers 0 Followers

Questions related from Archita Ghoshal

In RNA sequencing, Is it wrong to use just the p-value < 0.05 to determine significance, and not consider the q-value ?

If I have fold changes where the p-value is 0.05 and the q-value is about 0.6 or 0.7, can it be reported?

07 December 2017 6,098 7 View

Problem with glutathione agarose column for purification of mammalian protein expressed in E.coli - can anyone help?

I have cloned and expressed a mammalian protein in E.coli BL21 with GST tag. I use glutathione agarose beads (sigma) for affinity purification (not pre-packed column). I use 0.4% sarkosyl to...

14 November 2013 2,564 4 View

Expression of only GST protein in pGEX-4t2 containing gene of interest, instead of GST tagged fusion protein?

I have cloned my gene into pGEX-4t2 and expressed in E.coli BL21 DE3. However, my clones are expressing only GST protein. Sequencing results show that my gene has its correct sequence. Does that...

26 August 2013 9,752 10 View

How to analyze PDBsum results?

I docked 2 proteins and analyzed the docked structures using PDBsum. There is no literature stating any residues involved in the interaction. How do I draw a conclusion from the results I have...

30 October 2012 1,171 9 View