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I made a primer that gives 2 bands in every 15 samples in ZS11xSiloga BC3F1 genration. I thought that it might be technical problem, i again ccheck it with BC2F1 generation so i got homozygous...
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Actually, I am trying for months to make a primer of a Clubroot resistance gene (CRa). CRa gene is found in three Brassica species. My aim is to make a primer that can amplify this one gene in all...
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