April DeMell

2 Questions 2 Answers 0 Followers

Questions related from April DeMell

Why can I not get colonies when transforming E. coli with plasmid isolated from Agro?

I have Agro (GV3101 competent cells) with my plasmid that I want to isolate. I miniprepped from Agro using a plasmid DNA isolation kit, which yielded approximately 25-35 ng/ul plasmid. I tried to...

02 February 2019 4,387 6 View

What are the best conditions when using PuRe Taq ready-to-go- PCR beads?

I'm using the ready to go PCR beads for the first time. The protocol recommends a final volume of 25 uL. I tried using 2 uL of primer mix (1 uL of each primer, at a concentration of 5 uM, diluted...

07 July 2015 3,383 1 View