April DeMell

5 Questions 4 Answers 0 Followers

Questions related from April DeMell

Why is the GFP expressed from my viral vector so weak?

I made a plant viral vector expressing GFP. I got this GFP from a previously made construct (a viral vector of the same type) which I PCR amplified the GFP from, and used restriction enzyme...

20 October 2020 3,207 3 View

Nicotiana benthamiana growth chamber conditions?

UPDATE: SEE MY LAST COMMENT FOR SOLUTION! I'm trying to figure out the best conditions to grow my N. benthamiana plants in a growth chamber. I will be Agro infiltrating them later, around 4...

12 November 2019 6,775 10 View

Why can I not get colonies when transforming E. coli with plasmid isolated from Agro?

I have Agro (GV3101 competent cells) with my plasmid that I want to isolate. I miniprepped from Agro using a plasmid DNA isolation kit, which yielded approximately 25-35 ng/ul plasmid. I tried to...

28 February 2019 5,756 5 View

How might I deactivate a nuclease treatment?

I recently put a lysate through a nuclease treatment (DNase I and RNase A) before I intend to break open the phage capsids. Is there a way to deactivate the nuclease in the solution before...

17 July 2015 6,730 7 View

Can anyone share any fellowships, grants, or funding sources you know of for plant biology graduate students?

I'm trying to compile a list of funding opportunities for plant biology graduate students (for me, because I'm looking for funding, but also as a resource to share with others in the future). Does...

01 January 1970 7,325 3 View