Appy Mukherjee

5 Questions 8 Answers 0 Followers

Questions related from Appy Mukherjee

How to convert 2D stack images to 3D to obtain surface area of nucleus?

I have 15 slices of the nucleus at different z-heights from confocal imaging. I would like to use these slices to reconstruct the 3D nucleus and ultimately estimate the surface area of the...

21 February 2021 7,156 1 View

How to stain cells in 3D gels?

Hey everyone, I have done a lot of staining for cells on regular 2D surfaces (actin, microtubules, DAPI, etc). But I am now trying to stain cells that are embedded in 3D gels. I looked up papers...

22 July 2020 7,032 1 View

PDGF (Platelet Derived Growth Factor) Expiry Time?

I am using PDGF (https://www.fishersci.com/shop/products/thermo-scientific-pierce-pdgf-bb-human-recombinant-protein/pirp8657) for a chemotaxis assay. I would like to know how long I can use the...

21 July 2018 4,180 1 View

Shading Correction in ImageJ - Dividing or Subtracting Images?

While performing shading correction in ImageJ to remove local fluorescent light intensity variations due to camera issues, most sites recommend dividing the uncorrected image by the base image and...

06 June 2018 4,625 2 View

Is there a simple way to estimate the diffusion coefficient of a molecule in water from its molecular weight? (I don't know its viscosity)?

I know that the Stoke's relationship gives the relationship I am looking for but that requires me to know the viscosity of the molecule in the solvent right? I don't know that. It is a large...

23 May 2018 9,713 12 View