Appy Mukherjee

4 Questions 8 Answers 0 Followers

Questions related from Appy Mukherjee

How to convert 2D stack images to 3D to obtain surface area of nucleus?

I have 15 slices of the nucleus at different z-heights from confocal imaging. I would like to use these slices to reconstruct the 3D nucleus and ultimately estimate the surface area of the...

21 February 2021 7,130 1 View

How to stain cells in 3D gels?

Hey everyone, I have done a lot of staining for cells on regular 2D surfaces (actin, microtubules, DAPI, etc). But I am now trying to stain cells that are embedded in 3D gels. I looked up papers...

22 July 2020 7,025 1 View

PDGF (Platelet Derived Growth Factor) Expiry Time?

I am using PDGF (https://www.fishersci.com/shop/products/thermo-scientific-pierce-pdgf-bb-human-recombinant-protein/pirp8657) for a chemotaxis assay. I would like to know how long I can use the...

21 July 2018 4,172 1 View

Is there a simple way to estimate the diffusion coefficient of a molecule in water from its molecular weight? (I don't know its viscosity)?

I know that the Stoke's relationship gives the relationship I am looking for but that requires me to know the viscosity of the molecule in the solvent right? I don't know that. It is a large...

23 May 2018 9,706 12 View