Any one has experienced the issue that the medium didn't cover the whole area of 6-well plate during spin infection?

I recently transduced cells in a 6 well plate by spin infection. 1 mL of medium and 100 uL of concentrated lentivirus was put in the well. However, after I spin the plate with a speed of 1500 rpm...

15 July 2018 5,951 1 View