Aparna Pathak

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Questions related from Aparna Pathak

Sonication is not working for my yeast cells.Can I use Mnase digestion or is there any other alterrnative for sonication?any ?

I wanted to see localization of transcription factors as well as acetylation and methylation levels and for that I have to do chromatin immunoprecipitation followed by sequencing, and my...

3 weeks ago 4,455 1 View

Facing problems with sonication for chromatin immunoprecipitation assay for S. pombe yeast cells.What can be the possible reason?

Hello, I have been facing fragmentation problems with my Schizosacchoromyces pombe yeast samples that I need to use for ChIP assay.I add lysis buffer and glass beads to the pellet followed by...

09 July 2025 831 0 View