How to purify protein with si ilar PI as the ecoli proteins?

I'm trying to purify the TCF3 protein (~70-75kda kDa, pI 4.5) from E. coli BL21 cells after inducing expression with 1 mM IPTG. I am using Ni-NTA affinity chromatography to purify the His-tagged...

22 March 2025 1,783 3 View