Anwar Hussain

2 Questions 4 Answers 0 Followers

Questions related from Anwar Hussain

Has anyone observed degradation of transcript in IPTG induced Ecoli harboring vector with insert?

Any idea of degradation of Transcript from foreign gene in E. coli induced with IPTG to over express that gene.

03 March 2016 183 7 View

Is there any way to visualize if the first strand of cDNA has been synthesized without going for synthesizing second strand and doing PCR?

We have degraded RNA without any poly A tail from E. coli. We want to convert these RNA to cDNA and want to see if the strand has actually been synthesized? Size of these RNA ranges between 17-200...

02 February 2016 2,223 5 View