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My his tagged protein was expressed in BL21De3 RIPL cells with 1mM IPTG at 28C for 5 hours. Western blotting confirmed that my protein was expressing well. Hence, i went ahead with purification....
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Upon incubation of my protein with O/N with TEV protease (in 50mM Tris, 0.5mM EDTA), in the morning i saw a white thready precipitated formed. After running the SDS gels, following coomassie...
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