Anvesh Dasari

2 Questions 8 Answers 0 Followers

Questions related from Anvesh Dasari

His tag protein does not bind to Ni-column (new). What can i do to improve my protein binding?

My his tagged protein was expressed in BL21De3 RIPL cells with 1mM IPTG at 28C for 5 hours. Western blotting confirmed that my protein was expressing well. Hence, i went ahead with purification....

07 July 2016 5,997 7 View

Why does my protein precipitate when i add TEV protease to cleave the GST tag?

Upon incubation of my protein with O/N with TEV protease (in 50mM Tris, 0.5mM EDTA), in the morning i saw a white thready precipitated formed. After running the SDS gels, following coomassie...

06 June 2016 7,150 7 View