Anusree Mahanta

4 Questions 4 Answers 0 Followers

Questions related from Anusree Mahanta

Why does my background control always show higher absorbance values than samples in lipase activity assay?

Dear scientific colleagues I am performing lipase activity assay for Drosophila fat body (equivalent to human adipose tissue and liver). My issue is that my background control (sample homogenate...

26 February 2019 1,359 2 View

Can I use Applied Biosystems SYBR Green PCR mastermix with RT2 Profiler PCR array (Qiagen)?

Hi everyone I am planning to use the Qiagen RT2 Profiler PCR array for signaling pathways. Is it really necessary to use the RT2 SYBR Green Mastermixes and the RT2 First Strand Kit with the...

01 September 2016 8,391 5 View

Does Jurkat T cells express cytokines constitutively? In other words, are these lymphoma cells already activated cells?

I want to perform a time point analysis of expression of cytokines and signaling proteins in Jurkat T cells treated with a compound X. The confusion is whether these cells, being lymphomas have...

19 May 2016 3,610 5 View

Are Type 1 regulatory T (Tr1) cells the same as IFNgamma/IL-10 coproducing Th1 cells?

08 November 2014 7,451 0 View